Počet záznamů: 1

Interference with the 19S proteasomal regulatory complex subunit PSMD4 on the sperm surface inhibits sperm-zona pellucida penetration during porcine fertilization

  1. 1.
    0346865 - BTO-N 2011 RIV DE eng J - Článek v odborném periodiku
    Yi, Y.J. - Manandhar, G. - Sutovky, M. - Zimmerman, S.W. - Jonáková, Věra - Leeuwen van, F.W. - Oko, R. - Park, Ch.S. - Sutovsky, P.
    Interference with the 19S proteasomal regulatory complex subunit PSMD4 on the sperm surface inhibits sperm-zona pellucida penetration during porcine fertilization.
    Cell and Tissue Research. Roč. 341, č. 2 (2010), s. 325-340 ISSN 0302-766X
    Grant CEP: GA MŠk(CZ) 1M06011; GA ČR(CZ) GA303/09/1285; GA MZd(CZ) NS10009
    Výzkumný záměr: CEZ:AV0Z50520701
    Klíčová slova: Sperm * Proteasome * Fertilization * Zona pellucida * Acrosome * Alzheimer's * Porcine
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 2.804, rok: 2010

    Proteolysis of ubiquitinated sperm and oocyte proteins by the 26S proteasome is necessary for mammalian fertilization. The study examined the role of PSMD4, a subunit of 19S regulatory complex responsible for proteasome-substrate recognition, in sperm-ZP penetration during porcine fertilization in vitro (IVF). Immunofluorescence localized PSMD4 in the sperm acrosome. Immunoprecipitation and proteomic analysis revealed that PSMD4 co-precipitated with porcine sperm-associated acrosin inhibitor (AI). Ubiquitinated species of AI were isolated from boar sperm extracts by affinity purification of ubiquitinated proteins. Some proteasomes appeared to be anchored to the sperm head inner acrosomal membrane. In conclusion, the 19S regulatory complex subunit PSMD4 is involved in the sperm-ZP penetration during fertilization. The recognition of substrates on the ZP by the 19S proteasomal regulatory complex is essential for porcine/mammalian fertilization in vitro.
    Trvalý link: http://hdl.handle.net/11104/0187780