Počet záznamů: 1

Proviruses selected for high and stable expression of transduced genes accumulate in broadly transcribed genome areas

  1. 1.
    0346752 - UMG-J 2011 RIV US eng J - Článek v odborném periodiku
    Plachý, Jiří - Kotáb, Jan - Divina, Petr - Reinišová, Markéta - Šenigl, Filip - Hejnar, Jiří
    Proviruses selected for high and stable expression of transduced genes accumulate in broadly transcribed genome areas.
    Journal of Virology. Roč. 84, č. 9 (2010), s. 4204-4211 ISSN 0022-538X
    Grant CEP: GA ČR(CZ) GA204/07/1030; GA ČR GP301/09/P667
    Výzkumný záměr: CEZ:AV0Z50520514
    Klíčová slova: avian sarcoma virus * retrovirus integration * position effects
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 5.189, rok: 2010

    Retroviruses and retrovirus-derived vectors integrate non-randomly into the genomes of host cells with specific preferences for transcribed genes, gene-rich regions, and CpG islands. To understand the genomic features that influence transcriptinal activity of integrated retroviruses, we cloned and analyzed the integration sites of avian sarcoma virus from chicken tumors. Growing progressively in dependence on high and stable expression of the transduced v-src oncogene, these tumors represent clonal expansions of cells bearing transcriptionally active replication-defective proviruses. Our analysis showed striking accumulation within or close to transcription units, particularly in genes broadly expressed in multiple tissues but not in tissue-specifically expressed genes. We infer that proviruses integrated in these genomic areas efficiently avoid transcriptional silencing and keep active for a long time during the growth of tumors.
    Trvalý link: http://hdl.handle.net/11104/0187688
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