Počet záznamů: 1

Semisynthesis of C17:0 isoforms of sulphatide and glucosylceramide using immobilised sphingolipid ceramide N-deacylase for application in analytical mass spectrometry

  1. 1.
    0346664 - UMCH-V 2011 RIV GB eng J - Článek v odborném periodiku
    Kuchař, L. - Rotková, J. - Asfaw, B. - Lenfeld, Jiří - Horák, Daniel - Korecká, L. - Bílková, Z. - Ledvinová, J.
    Semisynthesis of C17:0 isoforms of sulphatide and glucosylceramide using immobilised sphingolipid ceramide N-deacylase for application in analytical mass spectrometry.
    Rapid Communications in Mass Spectrometry. Roč. 24, č. 16 (2010), s. 2393-2399 ISSN 0951-4198
    Grant CEP: GA ČR GA203/09/0857
    Výzkumný záměr: CEZ:AV0Z40500505
    Klíčová slova: reverse hydrolysis reaction * quantitative-determination * internal standards
    Kód oboru RIV: CB - Analytická chemie, separace
    Impakt faktor: 2.846, rok: 2010

    Sphingolipid ceramide N-deacylase (SCDase, EC 3.5.1.69) is a hydrolytic enzyme isolated from Psendomonas sp. TK 4. In addition to its primary deacylation function, this enzyme is able to reacylate lyso-sphingolipids under specific conditions. We immobilized the enzyme on magnetic macroporous cellulose and used it to semisynthesise C17:0 glucosylceramide and C17:0 sulphatide.
    Trvalý link: http://hdl.handle.net/11104/0005994