Počet záznamů: 1

Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels

  1. 1.
    0346383 - UMCH-V 2011 RIV DK eng J - Článek v odborném periodiku
    Stsiapanava, A. - Dohnálek, Jan - Gavira, J. A. - Kutý, Michal - Koudeláková, T. - Damborský, J. - Kutá-Smatanová, Ivana
    Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels.
    Acta Crystallographica Section D-Biological Crystallography. Roč. 66, č. 9 (2010), s. 962-969 ISSN 0907-4449
    Grant CEP: GA ČR GA310/09/1407; GA MŠk(CZ) LC06010
    Výzkumný záměr: CEZ:AV0Z40500505; CEZ:AV0Z60870520
    Klíčová slova: haloalkane dehalogenase DhaA * Rhodococcus rhodochrous * mutagenesis
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 6.326, rok: 2010

    Mutagenesis focused on the access tunnels of DhaA produced protein variants with significantly improved activity towards TCP. Three mutants of DhaA named DhaA04 (C176Y), DhaA14 (I135F) and DhaA15 (C176Y + I135F) were constructed in order to study the functional relevance of the enzyme tunnels The crystal structures of DhaA04, DhaA14 and DhaA15 with ligands present in the active site were solved and refined using diffraction data to 1.23, 0.95 and 1.22 Å, resolution, respectively. Structural comparisons of the wild type and the three mutants suggest that the tunnels play a key role in the processes of ligand exchange between the buried active site and the surrounding solvent.
    Trvalý link: http://hdl.handle.net/11104/0005975