Počet záznamů: 1

Conditions for succseful labeling of oxidation sensitive peptides by Na[125-I] - IODO-GEN(TM) system

  1. 1.
    0346291 - UOCHB-X 2011 RIV GB eng J - Článek v odborném periodiku
    Elbert, Tomáš - Veselá, Iva
    Conditions for succseful labeling of oxidation sensitive peptides by Na[125-I] - IODO-GEN(TM) system.
    Journal of Labelled Compounds and Radiopharmaceuticals. Roč. 53, 5/6 (2010), s. 288-291 ISSN 0362-4803.
    [International Symposium on the Synthesis and Applications of Isotopes and Isotopically Labelled Compounds /10./. Chicago, 14.06.2009-18.06.2009]
    Grant CEP: GA MŠk LA 288
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: Iodo-Gen * peptide * 125-I * radiolabelling
    Kód oboru RIV: CC - Organická chemie
    Impakt faktor: 1.096, rok: 2010

    Very good and reproducible yields of 125-I-labeled peptides containing tryptophan residue were achieved when the radioidination reaction was performed with 1 to 3 mCi of carrrier free Na[125-I] in 1.5 ml eppendorf PP tubes precoated with 10 nmols of IODO-GEN(TM) in phosphate buffer saline (pH=7.8). Addition of bovine serum albumin to the solutions of radiolabeled peptides greatly reduced the losses during their workup.
    Trvalý link: http://hdl.handle.net/11104/0187359