Počet záznamů: 1

Oligomerization of adenosin-5´-O-ylmethylphosphonate, an isopolar AMP analogue: Evaluation of the route to short oligoadenylates

  1. 1.
    0345878 - UOCHB-X 2011 RIV US eng J - Článek v odborném periodiku
    Pressová, Martina - Buděšínský, Miloš - Kóšiová, Ivana - Kopecký, V. Jr. - Cvačka, Josef - Kašička, Václav - Šimák, Ondřej - Točík, Zdeněk - Rosenberg, Ivan
    Oligomerization of adenosin-5´-O-ylmethylphosphonate, an isopolar AMP analogue: Evaluation of the route to short oligoadenylates.
    Biopolymers. Roč. 93, č. 3 (2010), s. 277-289 ISSN 0006-3525
    Grant CEP: GA MŠk(CZ) LC06077; GA MŠk(CZ) LC06061; GA AV ČR KAN200520801; GA ČR GA203/09/0820; GA ČR GA202/09/0193
    Grant ostatní: EMIL-FW6(XE) 503569
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: spontaneous oligomerization * oligoadenylates * phosphonate linkage
    Kód oboru RIV: CC - Organická chemie
    Impakt faktor: 2.572, rok: 2010

    In order to prepare a library of short oligoadenylate analogues featuring both the enzyme-stable internucleotide linkage and the 5´-O-methylphosphonate moiety and thus obtain a pool of potential RNase L agonists/antagonists, we studied spontaneous polycondensation of adenosin-5´-O-ylmethylphosphonic acid (pcA), an isopolar AMP analogue, and its imidazolide derivatives employing N,N´-dicyclohexylcarbodiimide under nonaqueousconditions and uranyl ions under aqueous conditions, resp. The RP LC-MS analyses of the reaction mixtures per se, and those obtained after the periodate treatment, along with analyses and separations by capillary zone electrophoresis, allowed for characterization of major linear and cyclic oligoadenylates obtained. Structure of selected compounds was supported, after their isolation, by NMR spectroscopy.
    Trvalý link: http://hdl.handle.net/11104/0187057