Počet záznamů: 1

PCR-DGGE-Based Study of Fecal Microbial Stability during the Long-Term Chitosan Supplementation of Humans

  1. 1.
    0345859 - UZFG-Y 2011 RIV CZ eng J - Článek v odborném periodiku
    Mrázek, Jakub - Koppová, Ingrid - Kopečný, Jan - Šimůnek, Jiří - Fliegerová, Kateřina
    PCR-DGGE-Based Study of Fecal Microbial Stability during the Long-Term Chitosan Supplementation of Humans.
    Folia Microbiologica. Roč. 55, č. 4 (2010), s. 352-358 ISSN 0015-5632
    Grant CEP: GA ČR(CZ) GA525/08/0803
    Výzkumný záměr: CEZ:AV0Z50450515
    Klíčová slova: Chitosan * Microbial stability * Microflora
    Kód oboru RIV: FB - Endokrinologie, diabetologie, metabolizmus, výživa
    Impakt faktor: 0.977, rok: 2010

    A feeding study was performed to monitor the effect of chitosan intake on the fecal microbiota of ten healthy human subjects. Diversity of microflora was monitored during 8 weeks including 4 weeks of chitosan supplementations. Using denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene amplicons and quantitative PCR method we revealed possible changes originating in the overall bacterial composition and also in the subpopulation of Bifidobacterium group. DGGE profiles displayed high complexity and individuality for each subject. Considerable variations in the composition of band patterns were observed among different persons. A raised level of fecal Bacteroides in response to chitosan intake was found in all samples. Bifidobacterium levels following chitosan intake increased or remain unchanged. Non-significant increase was, surprisingly, found in the numbers of butyrate-producing bacteria.
    Trvalý link: http://hdl.handle.net/11104/0187043
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