Počet záznamů: 1

New applications of pHluorin - measuring intracellular pH of prototrophic yeasts and determining changes in the buffering capacity of strains with affected potassium homeostasis

  1. 1.
    0345607 - FGU-C 2011 RIV GB eng J - Článek v odborném periodiku
    Marešová, Lydie - Hošková, B. - Urbánková, E. - Chaloupka, R. - Sychrová, Hana
    New applications of pHluorin - measuring intracellular pH of prototrophic yeasts and determining changes in the buffering capacity of strains with affected potassium homeostasis.
    Yeast. Roč. 27, č. 6 (2010), s. 317-325 ISSN 0749-503X
    Grant CEP: GA MŠk(CZ) LC531; GA AV ČR(CZ) KJB500110701
    Výzkumný záměr: CEZ:AV0Z50110509
    Klíčová slova: pH maintenance * Saccharomyces cerevisiae * fluorescence measurements
    Kód oboru RIV: ED - Fyziologie
    Impakt faktor: 1.626, rok: 2010

    We constructed a new pHluorin plasmid with the dominant selection marker (KanMX). This plasmid allows pH measurements in cells without auxotrophic mutations and/or grown in chemically indefinite media. We also utilised pHluorin in a new method of measuring the buffering capacity of yeast cell cytosol in vivo
    Trvalý link: http://hdl.handle.net/11104/0186841