Počet záznamů: 1

The use of superporous Ac-CGGASIKVAVS-OH-modified PHEMA scaffolds to promote cell adhesion and the differentiation of human fetal neural precursors

  1. 1.
    0345026 - UEM-P 2011 RIV NL eng J - Článek v odborném periodiku
    Kubinová, Šárka - Horák, Daniel - Kozubenko, Nataliya - Vaněček, Václav - Proks, Vladimír - Price, J. - Cocks, G. - Syková, Eva
    The use of superporous Ac-CGGASIKVAVS-OH-modified PHEMA scaffolds to promote cell adhesion and the differentiation of human fetal neural precursors.
    Biomaterials. Roč. 31, č. 23 (2010), s. 5966-5975 ISSN 0142-9612
    Grant CEP: GA MŠk(CZ) LC554; GA AV ČR(CZ) IAA500390902
    Grant ostatní:GA MŠk(CZ) 1M0538; GA ČR(CZ) GD309/08/H079; GA ČR(CZ) KAN200200651; GA ČR(CZ) GAP108/10/1560; EC FP6 project ARTEMIS(XE) LSHM-CT-2007-037862
    Program:1M; KA; GA
    Výzkumný záměr: CEZ:AV0Z50390703; CEZ:AV0Z40500505
    Klíčová slova: cell adhesion * laminin * mesenchymal stem cell
    Kód oboru RIV: FH - Neurologie, neurochirurgie, neurovědy
    Impakt faktor: 7.883, rok: 2010

    Modifications of poly(2-hydroxyethyl methacrylate) (PHEMA) with laminin-derived Ac-CGGASIKVAVS-OH peptide sequences have been developed to construct scaffolds that promote cell adhesion and neural differentiation. The Ac-CGGASIKVAVS-OH peptide was immobilized to the sulfhydryl groups by 2,2'-dithiodipyridine linking reagent via 2-[4-(2-pyridyldisulfanyl)butanamido]ethyl methacrylate. Superporous Ac-CGGASIKVAVS-OH-modified PHEMA scaffolds significantly increased the number of attached mesenchymal stem cells and their growth area on the hydrogel surface in the absence and in the presence of serum in the culture medium. Additionally, the Ac-CGGASIKVAVS-OH peptide supported the attachment, proliferation, differentiation and process spreading of human fetal neural stem cells during the first two weeks of expansion and contributed to the formation of a high percentage of more mature neural cells after four weeks of expansion.
    Trvalý link: http://hdl.handle.net/11104/0005938