Počet záznamů: 1

Crystallization and preliminary X-ray analysis of a novel haloalkane dehalogenase DbeA from Bradyrhizobium elkani USDA94

  1. 1.
    0343421 - UOCHB-X 2011 RIV GB eng J - Článek v odborném periodiku
    Prudnikova, T. - Mozga, T. - Řezáčová, Pavlína - Chaloupková, R. - Sato, Y. - Nagata, Y. - Brynda, Jiří - Kutý, Michal - Damborský, J. - Kutá-Smatanová, Ivana
    Crystallization and preliminary X-ray analysis of a novel haloalkane dehalogenase DbeA from Bradyrhizobium elkani USDA94.
    Acta Crystallographica Section F. F65, č. 4 (2009), s. 353-356 ISSN 1744-3091
    Grant CEP: GA MŠk(CZ) LC06010
    Výzkumný záměr: CEZ:AV0Z40550506; CEZ:AV0Z60870520; CEZ:AV0Z50520514
    Klíčová slova: protein crystallization * X-ray analysis * dehalogenase
    Kód oboru RIV: CC - Organická chemie
    Impakt faktor: 0.551, rok: 2009

    A novel enzyme, DbeA, belonging to the haloalkane dehalogenase family (EC 3.8.1.5) was isolated from Bradyrhizobium elkani USDA94. In order to understand the unique activity and specificity of DbeA, its mutant variant DbeA1, which carries the unique fragment of DbjA, was also constructed. Both wild-type DbeA and DbeA1 were crystallized using the sitting-drop vapour-diffusion method. The crystals of DbeA belonged to the primitive orthorhombic space group P2(1)2(1)2(1), while the crystals of DbeA1 belonged to the monoclinic space group C2. Diffraction data were collected to 2.2 A resolution for both DbeA and DbeA1 crystals.
    Trvalý link: http://hdl.handle.net/11104/0185903