Počet záznamů: 1

Crystallization and preliminary crystallographic characterization of the extrinsic PsbP protein of photosystem II from Spinacia oleracea

  1. 1.
    0343418 - UOCHB-X 2011 RIV GB eng J - Článek v odborném periodiku
    Kohoutová, Jaroslava - Kutá-Smatanová, Ivana - Brynda, Jiří - Lapkouski, Mikalai - Revuelta, J. L. - Arellano, J.B. - Ettrich, Rüdiger
    Crystallization and preliminary crystallographic characterization of the extrinsic PsbP protein of photosystem II from Spinacia oleracea.
    Acta Crystallographica Section F. F65, č. 2 (2009), s. 111-115 ISSN 1744-3091
    Grant CEP: GA MŠk(CZ) LC06010
    Výzkumný záměr: CEZ:AV0Z40550506; CEZ:AV0Z60870520
    Klíčová slova: photosystem protein * crystallization * X-ray analysis
    Kód oboru RIV: CC - Organická chemie
    Impakt faktor: 0.551, rok: 2009

    Preliminary X-ray diffraction analysis of the extrinsic PsbP protein of photosystem II from spinach (Spinacia oleracea) was performed using N-terminally His-tagged recombinant PsbP protein overexpressed in Escherichia coli. Recombinant PsbP protein (thrombin-digested recombinant His-tagged PsbP) stored in bis-Tris buffer pH 6.00 was crystallized using the sitting-drop vapour-diffusion technique with PEG 550 MME as a precipitant and zinc sulfate as an additive. SDS-PAGE analysis of a dissolved crystal showed that the crystals did not contain the degradation products of recombinant PsbP protein. PsbP crystals diffracted to 2.06 A resolution in space group P2(1)2(1)2(1).
    Trvalý link: http://hdl.handle.net/11104/0185901