Počet záznamů: 1

Surface modification of cyclic olefin copolymers for osteochondral defect repair can increase pro-destructive potential of human chondrocytes in vitro

  1. 1.
    0343392 - UFCH-W 2011 RIV CZ eng J - Článek v odborném periodiku
    Polanská, M. - Hulejová, H. - Petrtýl, M. - Bastl, Zdeněk - Spirovová, Ilona - Kruliš, Zdeněk - Horák, Zdeněk - Veigl, D. - Šenolt, L.
    Surface modification of cyclic olefin copolymers for osteochondral defect repair can increase pro-destructive potential of human chondrocytes in vitro.
    Physiological Research. Roč. 59, č. 2 (2010), s. 247-253 ISSN 0862-8408
    Grant CEP: GA ČR GA106/06/0761
    Výzkumný záměr: CEZ:AV0Z40400503; CEZ:AV0Z40500505
    Klíčová slova: osteochondral defects * cycloolefin copolymer * chondrocytes * biocompatibility
    Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
    Impakt faktor: 1.646, rok: 2010

    Materials on the basis of cycloolefin copolymers (COC) are suitable for subchondral defect repairs. The objective of this study was to evaluate the influence of surface modification of COC and COC/LLDPE blends on the viability and gene expression of chondrocytes. Human chondrocytes were incubated on the surface of the studied materials. Half of the materials were plasmatically modified with a subsequent type II collagen application. The gene expression of matrix metalloproteinases (MMP-1,-3,-13), pro-inflammatory cytokines (IL-1, TNF-alpha) and apoptotic molecules (BAX, Bcl-2) was evaluated using quantitative Taq-Man PCR after 48 h incubation. Chondrocyte viability was evaluated by the MTT test after 2, 4 and 8 days of incubation. The synthesis of MMPs was measured by ELISA assay in cell culture medium after 48 h of incubation.
    Trvalý link: http://hdl.handle.net/11104/0185883