Počet záznamů: 1

Mitochondrial membrane potential-based genome-wide RNAi screen of Trypanosoma brucei

  1. 1.
    0342948 - BC-A 2011 RIV DE eng J - Článek v odborném periodiku
    Verner, Zdeněk - Paris, Zdeněk - Lukeš, Julius
    Mitochondrial membrane potential-based genome-wide RNAi screen of Trypanosoma brucei.
    Parasitology Research. Roč. 106, č. 5 (2010), s. 1241-1244 ISSN 0932-0113
    Výzkumný záměr: CEZ:AV0Z60220518
    Klíčová slova: GENE-FUNCTION * INTERFERENCE * mitochondrion * SUBUNITS
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 1.812, rok: 2010

    We have screened the Trypanosoma brucei genome-wide RNAi library by staining the procyclics with the dye JC-1 followed by sorting the differentially stained cells by flow cytometry. This allowed us to highly enrich for cells in which mitochondrial membrane potential was decreased. We have further validated a subset of selected clones by a reverse approach in which we showed that cloning the selected genomic regions into another RNAi vector also results in a drop in mitochondrial membrane potential.
    Trvalý link: http://hdl.handle.net/11104/0185549