Počet záznamů: 1

Second harmonic generation from thick leaves using the two-photon laser scanning microscope

  1. 1.
    0342935 - UEK-B 2011 RIV GB eng J - Článek v odborném periodiku
    Reshak, Ali H
    Second harmonic generation from thick leaves using the two-photon laser scanning microscope.
    Micron. Roč. 4, č. 40 (2009), s. 455-462 ISSN 0968-4328
    Výzkumný záměr: CEZ:AV0Z60870520
    Klíčová slova: Duckweed Lemna minuta
    Kód oboru RIV: BO - Biofyzika
    Impakt faktor: 1.626, rok: 2009

    A laser-scanning microscope using Second harmonic generation (SHG) as a probe is shown to produce high-resolution images of duckweed Lemna minuta leaves. These leaves are multi-cell layer thick. Second harmonic light is generated by a tightly focused short pulse laser beam and is collected by two objectives yielding forward and backward second harmonic digital images. This measurement shows that the signal of the second harmonic imaging in the forward and backward directions depends on the thickness of the chloroplast and that the forward-SH image was brighter than the backward-SH image. The image intensity also depended on the orientation of the chloroplast in relation to the illuminating polarization direction. Their light-induced re-orientation which was affected by the intensity of the illumination could be observed during the experiments.
    Trvalý link: http://hdl.handle.net/11104/0185537