Počet záznamů: 1

Activation of protective cell-mediated immune response in gastric mucosa during Cryptosporidium muris infection and re-infection in immunocompetent mice

  1. 1.
    0342901 - BC-A 2011 RIV DE eng J - Článek v odborném periodiku
    Jalovecká, M. - Sak, Bohumil - Kváč, Martin - Květoňová, Dana - Kučerová, Z. - Salát, Jiří
    Activation of protective cell-mediated immune response in gastric mucosa during Cryptosporidium muris infection and re-infection in immunocompetent mice.
    Parasitology Research. Roč. 106, č. 5 (2010), s. 1159-1166 ISSN 0932-0113
    Grant CEP: GA AV ČR KJB500960701
    Výzkumný záměr: CEZ:AV0Z60220518
    Klíčová slova: Cryptosporidium muris * T-lymphocyte migration * gastric mucosa
    Kód oboru RIV: GJ - Choroby a škůdci zvířat, veterinární medicína
    Impakt faktor: 1.812, rok: 2010

    The immune response in the stomach mucosa during the primary infection and re-infection with Cryptosporidium muris (TS03 and CB03) in immunocompetent BALB/c mice was characterized using flow cytometry analysis and measurement of IFN-gamma and IL10 by enzyme-linked immunosorbent assays (ELISA). Significantly, elevated migration of T lymphocytes (more than 1,000-fold), especially CD8+ T lymphocytes, to the stomach mucosa occurred during primary infection and persisted for more than 2 months after its resolution. The ex vivo cultures of splenocytes revealed very low levels of IFN-gamma production during the course of the primary infection (0.5 ng/ml), whereas in the following re-exposure to the parasites, the concentration of IFN-gamma rapidly increased 22-fold. These results imply that the CD8+ T lymphocytes are involved in the immune response to gastric cryptosporidiosis and could play an important role in the elimination of C. muris infection in mice.
    Trvalý link: http://hdl.handle.net/11104/0185510