Počet záznamů: 1

Phosphomannose isomerase gene for selection in lettuce (Lactuca sativa L.) transformation

  1. 1.
    0342588 - BC-A 2011 RIV PL eng J - Článek v odborném periodiku
    Bříza, Jindřich - Růžičková, N. - Niedermeierová, Hana - Dusbábková, Jana - Vlasák, Josef
    Phosphomannose isomerase gene for selection in lettuce (Lactuca sativa L.) transformation.
    Acta Biochimica Polonica. Roč. 57, č. 1 (2010), s. 63-68 ISSN 0001-527X
    Výzkumný záměr: CEZ:AV0Z50510513
    Klíčová slova: pmi * npt II * Lactuca sativa
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 1.234, rok: 2010

    A positive selection system using phosphomannose isomerase was employed for Agrobacterium tumefaciens mediated transformation of lettuce (Lactuca sativa L. var. ‘Achát’). It was shown that the mannose-based selection system works very well with the lettuce genotype used, reaching up to 25 % transformation efficiency on the medium with 20 g/L mannose and 20 g/L sucrose.
    Trvalý link: http://hdl.handle.net/11104/0185286