Počet záznamů: 1

Selective binding of tumor suppressor p53 protein to topologically constrained DNA: Modulation by intercalative drugs

  1. 1.
    0342523 - BFU-R 2011 RIV NL eng J - Článek v odborném periodiku
    Pivoňková, Hana - Šebest, Peter - Pečinka, P. - Tichá, Olga - Němcová, Kateřina - Brázdová, Marie - Brázdová Jagelská, Eva - Brázda, Václav - Fojta, Miroslav
    Selective binding of tumor suppressor p53 protein to topologically constrained DNA: Modulation by intercalative drugs.
    Biochemical and Biophysical Research Communications. Roč. 393, č. 4 (2010), s. 894-899 ISSN 0006-291X
    Grant CEP: GA AV ČR(CZ) IAA500040701; GA ČR(CZ) GP204/07/P476; GA ČR(CZ) GP301/07/P160; GA AV ČR(CZ) 1QS500040581; GA MŠk(CZ) LC06035; GA ČR(CZ) GA204/08/1560
    Výzkumný záměr: CEZ:AV0Z50040507; CEZ:AV0Z50040702
    Klíčová slova: p53-DNA binding * supercoiled DNA * DNA topology
    Kód oboru RIV: BO - Biofyzika
    Impakt faktor: 2.595, rok: 2010

    Selective binding of p53 to negatively and positively supercoiled DNA was studied using intercalative drugs chloroquine, ethidium bromide, acridine derivatives and doxorubicin as a modulators of the level of DNA supercoiling. The p53 was found to lose gradually its preferential binding to negatively scDNA with increasing concentrations of intercalators until the DNA negative superhelix turns were relaxed. Formation of positive superhelices rendered the circular duplex DNA to be preferentially bound by the p53 again.
    Trvalý link: http://hdl.handle.net/11104/0185238