Počet záznamů: 1

Synthesis of nucleoside and nucleotide conjugates of bile acids, and polymerase construction of bile acid-functionalized DNA

  1. 1.
    0342359 - UOCHB-X 2011 RIV GB eng J - Článek v odborném periodiku
    Ikonen, Satu - Macíčková-Cahová, Hana - Pohl, Radek - Šanda, Miloslav - Hocek, Michal
    Synthesis of nucleoside and nucleotide conjugates of bile acids, and polymerase construction of bile acid-functionalized DNA.
    Organic & Biomolecular Chemistry. Roč. 8, č. 5 (2010), s. 1194-1201 ISSN 1477-0520
    Grant CEP: GA MŠk LC512; GA ČR GA203/09/0317
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: steroids * nucleosides * nucleoside triphosphates * DNA polymerase
    Kód oboru RIV: CC - Organická chemie
    Impakt faktor: 3.451, rok: 2010

    Sonogashira cross-coupling reactions of 5-iodopyrimidine or 7-iodo-7-deazaadenine nucleosides with bile acid-derived terminal acetylenes gave the corresponding bile acid-nucleoside conjugates. Analogous reactions of halogenated nucleoside triphosphates gave directly bile acid-modified dNTPs. Enzymatic incorporation of these modified nucleotides to DNA was successfully performed using Phusion polymerase for primer extension.
    Trvalý link: http://hdl.handle.net/11104/0185111