Detection of condensin I and II in maturing pig oocytes

0341856 - ÚŽFG 2011 RIV AU eng J - Článek v odborném periodiku
Lišková, Lucie - Šušor, Andrej - Pivoňková, Kateřina - Šašková, Adéla - Karabínová, Pavla - Kubelka, Michal
Detection of condensin I and II in maturing pig oocytes.
Reproduction Fertility and Development. Roč. 22, č. 4 (2010), s. 644-652. ISSN 1031-3613. E-ISSN 1448-5990
Grant CEP: GA ČR GA204/06/1297; GA ČR(CZ) GD204/09/H084; GA ČR GP524/09/P435
Výzkumný záměr: CEZ:AV0Z50450515
Klíčová slova: Chromosome condensation * Female meiosis * Phosphorylation
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.553, rok: 2010

The multiprotein complexes known as condensins (I and II) are major players in chromosome dynamics in mitotic and meiotic cells. Here, we report for the first time the detection of different condensin subunits from both complexes in mammalian oocytes. Using immunoblotting analysis we examined expression levels of condensin subunits during meiotic maturation of porcine oocytes. The expression of the core subunit structural maintenance of chromosomes 2 (SMC2), identical in both condensin complexes, did not change significantly during maturation. Similarly, there was no significant change in the expression of the chromosome associated protein (CAP)-H and CAP-H2 subunits, components of condensin I and II, respectively. Conversely, the expression profiles of CAP-G, CAP-D2 (condensin I) and CAP-D3 (condensin II) were more interesting. At least two isoforms of the CAP-D2 subunit were detected, along with three isoforms of the CAP-D3 and CAP-G subunits.
Trvalý link: http://hdl.handle.net/11104/0184715