Počet záznamů: 1

Effect of hydrophobic interactions on properties and stability of DNA-polyelectrolyte complexes

  1. 1.
    0341720 - UMCH-V 2011 RIV US eng J - Článek v odborném periodiku
    Filippov, Sergey K. - Koňák, Čestmír - Kopečková, P. - Starovoytova, Larisa - Špírková, Milena - Štěpánek, Petr
    Effect of hydrophobic interactions on properties and stability of DNA-polyelectrolyte complexes.
    Langmuir. Roč. 26, č. 7 (2010), s. 4999-5006 ISSN 0743-7463
    Grant CEP: GA ČR GA202/09/2078
    GRANT EU: European Commission(XE) 512087 - GIANT
    Výzkumný záměr: CEZ:AV0Z40500505
    Klíčová slova: DNA * polyplex * cholesterol
    Kód oboru RIV: BO - Biofyzika
    Impakt faktor: 4.269, rok: 2010

    Polyplexes are polyelectrolyte complexes of DNA and polycations, designed for potential gene delivery. We investigated the properties of new polyplexes formed from cholesterol-modified polycations and DNA. Three complexes were tested; their cholesterol contents were 1.4, 6.3, and 8.7 mol %. UV spectroscopy and fluorescence assay using ethidium bromide proved the formation of polyplexes. The kinetics of turbidity of polyplexes solutions in physiological solution showed that the colloid stability of polyplexes increases with increasing content of cholesterol in polycations. DLS, SLS, electrophoretic light scattering, SAXS, and atomic force microscopy were used for characterization of polyplexes. The observed hydrodynamic radii of polyplexes were in the range of 30-60 nm. The properties of polyplex particles depend, in addition to polycation structure, on the rate of polycation addition to DNA solutions.
    Trvalý link: http://hdl.handle.net/11104/0005836