Počet záznamů: 1

Expression, purification and preliminary crystallization study of RpaC protein from Synechocystis sp PCC6803

  1. 1.
    0341135 - UEK-B 2013 RIV NL eng J - Článek v odborném periodiku
    Cséfalvay, Eva - Lapkouski, Mikalai - Komárek, Ondřej
    Expression, purification and preliminary crystallization study of RpaC protein from Synechocystis sp PCC6803.
    Photosynthetica. Roč. 3, č. 47 (2009), s. 355-362 ISSN 0300-3604
    Grant CEP: GA ČR GA206/07/0917
    Výzkumný záměr: CEZ:AV0Z60870520
    Klíčová slova: affinity chromatography * photosystem * phycobilisome – PSII supercomplex
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 1.072, rok: 2009

    State transitions in cyanobacteria are physiological adaptation mechanisms that change the interaction of the phycobilisomes with the photosystem I and photosystem II core complexes. Previous studies of cyanobacteria have identified a gene named rpaC, which appears to be specifically required for state transitions. The gene product of rpaC is very probably a transmembrane protein that is a structural component of the phycobilisome-photosystem II supercomplex. Here we report the construction of an expression system that enables high production of fusion protein TrxHisTagSTag-RpaC, and describe suitable conditions for purification of this insoluble protein at a yield of 3 mg per 1 dm3 of bacterial culture.
    Trvalý link: http://hdl.handle.net/11104/0184214