Počet záznamů: 1
Expression, purification and preliminary crystallization study of RpaC protein from Synechocystis sp PCC6803
0341135 - UEK-B 2013 RIV NL eng J - Článek v odborném periodiku
Cséfalvay, Eva - Lapkouski, Mikalai - Komárek, Ondřej
Expression, purification and preliminary crystallization study of RpaC protein from Synechocystis sp PCC6803.
Photosynthetica. Roč. 3, č. 47 (2009), s. 355-362 ISSN 0300-3604
Grant CEP: GA ČR GA206/07/0917
Výzkumný záměr: CEZ:AV0Z60870520
Klíčová slova: affinity chromatography * photosystem * phycobilisome – PSII supercomplex
Kód oboru RIV: CE - Biochemie
Impakt faktor: 1.072, rok: 2009
State transitions in cyanobacteria are physiological adaptation mechanisms that change the interaction of the phycobilisomes with the photosystem I and photosystem II core complexes. Previous studies of cyanobacteria have identified a gene named rpaC, which appears to be specifically required for state transitions. The gene product of rpaC is very probably a transmembrane protein that is a structural component of the phycobilisome-photosystem II supercomplex. Here we report the construction of an expression system that enables high production of fusion protein TrxHisTagSTag-RpaC, and describe suitable conditions for purification of this insoluble protein at a yield of 3 mg per 1 dm3 of bacterial culture.
Trvalý link: http://hdl.handle.net/11104/0184214