Počet záznamů: 1

Fetal colon cell line FHC exhibits tumorigenic phenotype, complex karyotype, and TP53 gene mutation

  1. 1.
    0340999 - BFU-R 2010 RIV US eng J - Článek v odborném periodiku
    Souček, Karel - Jirsová, Pavla - Brázdová, Marie - Hýžďalová, Martina - Kočí, Lenka - Vydra, D. - Trojanec, R. - Pernicová, Zuzana - Lentvorská, L. - Hajdúch, M. - Hofmanová, Jiřina - Kozubík, Alois
    Fetal colon cell line FHC exhibits tumorigenic phenotype, complex karyotype, and TP53 gene mutation.
    Cancer Genetics and Cytogenetics. Roč. 197, č. 2 (2010), s. 107-116 ISSN 0165-4608
    Grant CEP: GA MŠk ME 919; GA ČR(CZ) GA204/07/0834; GA ČR(CZ) GA204/08/1560; GA AV ČR(CZ) 1QS500040507; GA MZd NS9600
    Výzkumný záměr: CEZ:AV0Z50040507; CEZ:AV0Z50040702
    Klíčová slova: colon epithelial cells * TP53 * MYC
    Kód oboru RIV: BO - Biofyzika
    Impakt faktor: 1.551, rok: 2010

    The FHC (fetal human cells) cell line has been established from normal fetal colonic mucosa. Detailed characterization of this cell line and mechanism of spontaneously acquired immortality have not been described yet. Therefore, we characterized the FHC cell line in terms of its tumorigenicity, cytogenetics, and TP53 gene mutation analysis. FHC cells displayed capability for anchorage-independent growth in semisolid media in vitro and formed solid tumors after transplantation into SCID (severe combined immunodeficiency) mice. This tumorigenic phenotype was associated with hypotriploidy and chromosome number ranging from 66 to 69. We identified mutation of TP53 gene in codon 273; triplet CGT coding Arg was changed to CAG coding His. Because of their origin from normal fetal colon and their relative resistance to the induction of apoptosis, FHC cells can be considered a valuable experimental model for various studies.
    Trvalý link: http://hdl.handle.net/11104/0184117