Počet záznamů: 1

Mutasynthesis of lincomycin derivatives with activity against drug-resistant staphylococci

  1. 1.
    0340856 - MBU-M 2010 RIV US eng J - Článek v odborném periodiku
    Ulanová, Dana - Novotná, Jitka - Smutná, Yvona - Kameník, Zdeněk - Gažák, Radek - Šulc, Miroslav - Sedmera, Petr - Kadlčík, Stanislav - Plháčková, Kamila - Janata, Jiří
    Mutasynthesis of lincomycin derivatives with activity against drug-resistant staphylococci.
    Antimicrobial Agents and Chemotherapy. Roč. 54, č. 2 (2010), s. 927-930 ISSN 0066-4804
    Grant CEP: GA MŠk 2B08064; GA AV ČR IAA500200810
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: COAGULASE-NEGATIVE STAPHYLOCOCCI * ERYTHROMYCIN RESISTANCE * SUBSTRATE-SPECIFICITY
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 4.672, rok: 2010

    The lincomycin biosynthetic gene lmbX was deleted in Streptomyces lincolnensis ATCC 25466 that led to abolition of lincomycin production. Complementation experiments proved the blockage in biosynthesis of lincomycin precursor 4-propyl-L proline. Feeding this mutant strain with precursor derivatives resulted in production of 4-butyl-4-depropyllincomycin and 4-pentyl-4-depropyllincomycin in high titers and without lincomycin contamination. Moreover, 4-pentyl-4-depropyllincomycin was found to be more active than lincomycin against clinical Staphylococcus isolates with genes determining low-level lincosamide resistance
    Trvalý link: http://hdl.handle.net/11104/0184015