Počet záznamů: 1

Condensation reactions catalyzed by α-N-acetylgalactosaminidase from Aspergillus niger yielding a-N-acetylgalactosaminides

  1. 1.
    0340836 - MBU-M 2010 RIV GB eng J - Článek v odborném periodiku
    Weignerová, Lenka - Pelantová, Helena - Manglová, Dana - Michálková, Klára - Křen, Vladimír
    Condensation reactions catalyzed by α-N-acetylgalactosaminidase from Aspergillus niger yielding a-N-acetylgalactosaminides.
    Biocatalysis and Biotransformation. Roč. 28, č. 2 (2010), s. 150-155 ISSN 1024-2422
    Grant CEP: GA MŠk OC 136
    Klíčová slova: alpha-N-Acetylgalactosaminidase * amino acid glycosylation * enzymatic glycosylation
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 1.275, rok: 2010

    Extracellular a-N-acetylgalactosaminidase from Aspergillus niger catalyzed glycosylation yielding a series of 2-acetamido-2-deoxy-a-D-galactobiosides using 2-acetamido-2-deoxy-D-galactopyranose as a glycosyl donor. The isomers a-D-GalpNAc-D-GalpNAc, a-D-GalpNAc-D-GalpNAc and a-D-GalpNAc-D-GalfNAc were isolated and spectrally characterized. The purified enzyme was further used for the glycosylation of free amino acids (serine and threonine) and their N-(tert-butoxycarbonyl)-protected analogs to synthesize the Tn antigen (GalpNAc-a-O-Ser/Thr) and its N-(tertbutoxycarbonyl)-protected derivatives
    Trvalý link: http://hdl.handle.net/11104/0183998