Počet záznamů: 1

Plant Ribonucleases and Nucleases as Antiproliferative Agens Targeting Human Tumors Growing in Mice

  1. 1.
    0340102 - BC-A 2010 RIV NL eng J - Článek v odborném periodiku
    Matoušek, Jaroslav - Matoušek, Josef
    Plant Ribonucleases and Nucleases as Antiproliferative Agens Targeting Human Tumors Growing in Mice.
    Recent Patents on DNA & Gene Sequences. Roč. 4, č. 1 (2010), s. 29-39 ISSN 1872-2156
    Grant CEP: GA ČR GA521/06/1149; GA ČR GA521/09/1214
    Výzkumný záměr: CEZ:AV0Z50510513; CEZ:AV0Z50450515
    Klíčová slova: antiproliferative cytotoxic * effect human * plant nuclease
    Kód oboru RIV: EB - Genetika a molekulární biologie

    Recombinant plant nucleases of tomato (TBN1) and hop (HBN1) (submitted to patenting under no. PV 2008-384;Z7585) were isolated to homogeneity and examined for their antitumor effects and cytotoxicity. Although antiproliferative effects of both recombinant nucleases were not significant on the ML-2 cell culture in vitro, the nucleases were strongly cytostatic in vivo after their administration intravenously as stabilized conjugates with polyethylene glycol (PEG). However, the antitumor effect of the first nuclease tested, the mung bean nuclease (PhA), on ML2 human tumor cell line was neither effective, even when significant antitumor activity was detected on human melanoma tumors in vivo. Recombinant TBN1 and HBN1 were as effective against human melanoma tumors as previously studied pine pollen nuclease and PhA and their effects were reached at about ten times lower concentrations compared to the use of bovine seminal RNase (BS-RNase).
    Trvalý link: http://hdl.handle.net/11104/0183420