Počet záznamů: 1

Identification of Rhodococcus fascians cytokinins and their modus operandi to reshape the plant

  1. 1.
    0337284 - UEB-Q 2010 RIV US eng J - Článek v odborném periodiku
    Pertry, I. - Václavíková, Kateřina - Depuydt, S. - Galuszka, P. - Spíchal, Lukáš - Temmerman, W. - Stes, E. - Schmülling, T. - Kakimoto, T. - Van Montagu, M. C. E. - Strnad, Miroslav - Holsters, M. - Tarkowski, Petr - Vereecke, D.
    Identification of Rhodococcus fascians cytokinins and their modus operandi to reshape the plant.
    Proceedings of the National Academy of Sciences of the United States of America. Roč. 106, č. 3 (2009), s. 929-934 ISSN 0027-8424
    Výzkumný záměr: CEZ:AV0Z50380511
    Klíčová slova: phytopathogen * actinomycete * phytohormone
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 9.432, rok: 2009

    Decades ago, the importance of cytokinins (CKs) during Rhodococcus fascians pathology had been acknowledged, and an isopentenyltransferase gene had been characterized in the fas operon of the linear virulence plasmid, but hitherto, no specific CK(s) could be associated with virulence. We show that the CK receptors AHK3 and AHK4 of Arabidopsis thaliana are essential for symptom development, and that the CK perception machinery is induced upon infection, underlining its central role in the symptomatology. Three classical CKs [ isopentenyladenine, trans-zeatin, and cis-zeatin (cZ)] and their 2-methylthio (2MeS)-derivatives were identified by CK profiling of both the pathogenic R. fascians strain D188 and its nonpathogenic derivative D188-5. However, the much higher CK levels in strain D188 suggest that the linear plasmid is responsible for the virulence-associated production. All R. fascians CKs were recognized by AHK3 and AHK4, and, although they individually provoked typical CK responses.
    Trvalý link: http://hdl.handle.net/11104/0181313