Počet záznamů: 1

Extraction and separation of water soluble proteins from Bacillus thuringiensis-transgenic and non-transgenic maize species by CZE

  1. 1.
    0336576 - UOCHB-X 2010 RIV DE eng J - Článek v odborném periodiku
    Sázelová, Petra - Kašička, Václav - Ibanez, E. - Cifuentes, A.
    Extraction and separation of water soluble proteins from Bacillus thuringiensis-transgenic and non-transgenic maize species by CZE.
    Journal of Separation Science. Roč. 32, č. 21 (2009), s. 3801-3808 ISSN 1615-9306
    Grant CEP: GA ČR(CZ) GA203/08/1428
    Grant ostatní: GA ČR(CZ) GA203/09/0675
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: Bacillus thuringiensis-transgenic maize * CZE-UV profiling * Maize proteins
    Kód oboru RIV: CB - Analytická chemie, separace
    Impakt faktor: 2.551, rok: 2009

    A new method for extraction and capillary electrophoretic (CE) separation and characterization of water-soluble proteins (albumin fraction) from Bacillus thuringiensis-transgenic and non-transgenic maize varieties was developed. The best CE separation of extracted proteins was achieved in isoelectric background electrolyte (200mM iminodiacetic acid, pH 2.26). Well resolved components of protein extracts were characterized by effective electrophoretic mobilities and corrected peak areas. Significant qualitative and quantitative differences in CE-UV profiling were found, which could potentially be used to distinguish the B. thuringiensis variety from the wild maize species.
    Trvalý link: http://hdl.handle.net/11104/0180780