Počet záznamů: 1

DNA vaccination with all-trans retinoic acid treatment induces long-term survival and elicits specific immune responses requiring CD4+ and CD8+ T-cell activation in an acute promyelocytic leukemia mouse model

  1. 1.
    0335722 - UMG-J 2010 RIV US eng J - Článek v odborném periodiku
    Furugaki, K. - Pokorná, Kateřina - le Pogam, C. - Aoki, M. - Reboul, M. - Bajzik, V. - Krief, P. - Janin, A. - Noguera, M.-E. - West, R. - Charron, D. - Chomienne, C. - Pla, M. - Moins-Teisserenc, H. - Padua, R.A.
    DNA vaccination with all-trans retinoic acid treatment induces long-term survival and elicits specific immune responses requiring CD4+ and CD8+ T-cell activation in an acute promyelocytic leukemia mouse model.
    Blood. Roč. 115, č. 3 (2010), s. 653-656 ISSN 0006-4971
    Grant ostatní: GA UK(CZ) 94308
    Výzkumný záměr: CEZ:AV0Z50520514
    Klíčová slova: all-trans retinoic acid * DNA vaccination * protective immunity
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 10.558, rok: 2010

    DNA vaccination and all-trans retinoic acid (ATRA) bring survival advantage in a mouse model of acute promyelocytic leukemia (APL). Depletion of CD4+ or CD8+ cells abolished this effect. CD4+ depletions of long-term survivors resulted in relapse and death within 3 months, thus demonstrating need of both cell subsets for generation of DNA-driven antileukemic immune responses and underscoring a crucial role of CD4+ cells in maintenance of durable remissions. Degranulation and cytotoxic carboxyfluorescein diacetate succinimidyl ester-based assays showed MHC-restricted APL-specific T cell-mediated immune responses. Sorted APL-specific CD8+CD107a+ T cells showed increased antileukemic activity. Effectors from ATRA + DNA-treated mice were shown to secrete IFN-g when stimulated with APL cells or peptides from the PL-RARa vaccine-derived sequences as shown by ELISpot assays. DNA vaccination with ATRA thus confers effective boosting of IFN-g-producing and cytotoxic T cells in leukemic mice.
    Trvalý link: http://hdl.handle.net/11104/0180110