Počet záznamů: 1

Proving tumour cells by acute nutritional/energy deprivation as a survival threat: a task for microscopy

  1. 1.
    0334131 - UMG-J 2010 RIV GR eng J - Článek v odborném periodiku
    Janečková, H. - Veselý, Pavel - Chmelík, R.
    Proving tumour cells by acute nutritional/energy deprivation as a survival threat: a task for microscopy.
    Anticancer Research. Roč. 29, č. 6 (2009), s. 2339-2345 ISSN 0250-7005
    Výzkumný záměr: CEZ:AV0Z50520514
    Klíčová slova: tumour cell * nutritional deprivation * energy deprivation * cell survival * cell death * digital holographic microscopy * dynamic phase difference
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 1.428, rok: 2009

    Malignant cells appear to possess a special aptitude for survival. We attempted to prove this in vitro by acute nutritional and energy deprivation as a survival threat. A PBS survival test in cell culture allowed static observations. These were supplemented by classic and quantitative phase-contrast time-lapse microscopy. From one normal and four neoplastic cell populations, no cells survived 77 hours of exposure to PBS. Only G3S2 derived from a human breast carcinoma survived 60 hrs. Cells in sparse culture were more vulnerable than those in dense. Epithelial cells were more vigorous than mesenchymal. Cells of greater malignancy resisted longer. Evaluation in culture as detailed by digital holographic microscopy (DHM) revealed increased compactness of the intracellular mass motility from normal to metastasizing mesenchymal cells, thus reaching the level of epithelial G3S2 cells. PBS survival test with DHM opens a new approach to studying the structural integrity of neoplastic cells.
    Trvalý link: http://hdl.handle.net/11104/0178947