Počet záznamů: 1

Silencing CENPF in bovine preimplantation embryo induces arrest at 8-cell stage

  1. 1.
    0334003 - UZFG-Y 2010 RIV GB eng J - Článek v odborném periodiku
    Toralová, Tereza - Šušor, Andrej - Němcová, Lucie - Kepková, Kateřina - Kaňka, Jiří
    Silencing CENPF in bovine preimplantation embryo induces arrest at 8-cell stage.
    Reproduction. Roč. 138, č. 5 (2009), s. 783-791 ISSN 1470-1626
    Grant CEP: GA ČR GA523/06/1226; GA ČR(CZ) GD204/05/H023
    Výzkumný záměr: CEZ:AV0Z50450515
    Klíčová slova: CENPF * bovine embryo * preimplantation development
    Kód oboru RIV: GI - Šlechtění a plemenářství hospodářských zvířat
    Impakt faktor: 2.579, rok: 2009

    The paper deals with the role of CENPF/mitosin during preimplantation development of cattle. We focused on monitoring of developmental competence of bovine embryos after injection of CENPF dsRNA into the zygotes. An average decrease of CENPF mRNA abundance by 94.9% or more and an extensive decline in immunofluorescence staining intensity was detected relative to controls. There was no disparity between the groups in the developmental competence before the 8-cell stage, where embryonic genome activation (EGA) in bovines takes place. However, the developmental competence rapidly decreased then and only 28.1% of CENPF dsRNA injected 8-cell embryos were able to develop further (controls 72.0%). We have further shown that in bovine post-EGA embryos CENPF protein is expressed in the same manner as in somatic cells. However, the protein in pre-EGA embryos is likely not degraded at the end of cell cycle, which enables the development of embryos till 8-cell stage.
    Trvalý link: http://hdl.handle.net/11104/0178848