Počet záznamů: 1

Dazap2 modulates transcription driven by the Wnt effector TCF-4

  1. 1.
    0333973 - UMG-J 2010 RIV GB eng J - Článek v odborném periodiku
    Lukáš, Jan - Mazna, Petr - Valenta, Tomáš - Doubravská, Lenka - Pospíchalová, Vendula - Vojtěchová, Martina - Fafílek, Bohumil - Ivánek, Robert - Plachý, Jiří - Novák, Jakub - Kořínek, Vladimír
    Dazap2 modulates transcription driven by the Wnt effector TCF-4.
    Nucleic Acids Research. Roč. 37, č. 9 (2009), s. 3007-3020 ISSN 0305-1048
    Grant CEP: GA ČR(CZ) GA204/07/1567; GA MŠk 2B06077
    Výzkumný záměr: CEZ:AV0Z50520514
    Klíčová slova: Wnt signaling * Tcf/Lef transcription factors * Dazap2/Prtb * transcriptional regulation
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 7.479, rok: 2009

    A typical feature of the transcriptional response induced by Wnt signaling is the involvement of Tcf/Lef factors that function in the nucleus as the principal mediators of signaling. Vertebrate Tcf/Lef proteins perform two well-characterized functions: In association with beta-catenin they activate gene expression, and in the absence of Wnt ligands they act as transcriptional repressors. In this report we reveal that the evolutionary conserved Dazap2 protein functions as a TCF-4 interacting partner. We demonstrate that a short region proximal to the TCF-4 HMG box mediates the interaction and that all Tcf/Lef family members associate with Dazap2. Interestingly, knockdown of Dazap2 not only reduced the activity of Wnt signaling as measured by Tcf/beta-catenin reporters but additionally altered the expression of Wnt signaling target genes. Finally, chromatin immunoprecipitation studies indicate that Dazap2 modulates the affinity of TCF-4 for its DNA recognition motif.
    Trvalý link: http://hdl.handle.net/11104/0178824