Počet záznamů: 1

Compensation of illumination inhomogeneities in multi-resolution image acquisition in confocal microscopy

  1. 1.
    0333693 - FGU-C 2010 RIV RU eng C - Konferenční příspěvek (zahraniční konf.)
    Tomori, Z. - Čapek, Martin - Michálek, Jan - Kubínová, Lucie
    Compensation of illumination inhomogeneities in multi-resolution image acquisition in confocal microscopy.
    [Kompenzace jasových nehomogenit při snímání obrazů konfokální mikroskopií.]
    GraphiCon´2009. Moscow: Lomonosov Moscow State University, 2009, s. 108-111.
    [International Conference on Computer Graphics and Vision /19./. Moscow (RU), 05.10.2009-09.10.2009]
    Grant CEP: GA ČR(CZ) GA102/08/0691; GA MŠk(CZ) MEB080831
    Výzkumný záměr: CEZ:AV0Z50110509
    Klíčová slova: confocal microscopy * light attenuation * distribution function
    Kód oboru RIV: IN - Informatika

    A multi-resolution application in modern confocal microscopy is based on a set of objectives with different resolution and motorized XY stage. An image captured through a low-resolution objective serves as navigation map where the user points to positions or areas of interest in which a high-resolution image will be captured. This allows e.g. automated acquisition in time lapse experiments, generating random, or systematic random positions for stereological counting frames, image tiling etc. However, captured image series representing a volume of interest suffers from illumination inhomogeneities. Algorithms described in this paper compensate both uneven illumination of captured images and light attenuation with depth. The first problem was solved by using modified morphological operation called the upper Lipschitz cover. To compensate the light attenuation with depth, a novel, very fast algorithm based on matching of distribution functions has been proposed

    Popisujeme metodu pro kompenzaci jasových nehomogenit obrazů nasnímaných pomocí konfokální mikroskopie
    Trvalý link: http://hdl.handle.net/11104/0178623