Počet záznamů: 1

Mapping of HIV-1 integrase preferences for target site selection with various oligonucleotides

  1. 1.
    0333523 - UOCHB-X 2010 RIV US eng J - Článek v odborném periodiku
    Snášel, Jan - Rosenberg, Ivan - Pačes, Ondřej - Pichová, Iva
    Mapping of HIV-1 integrase preferences for target site selection with various oligonucleotides.
    Archives of Biochemistry and Biophysics. Roč. 488, č. 2 (2009), s. 153-162 ISSN 0003-9861
    Grant CEP: GA MŠk 1M0508; GA MŠk(CZ) LC06077
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: HIV integrase * strand transfer reaction * oligonucleotide substrates
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 3.046, rok: 2009

    HIV integrase (IN) catalyzes the insertion of proviral DNA into the host cell chromosome. The integration site preference has been shown to be very diverse. We mapped the HIV integrase strand transfer reaction requirements using various short oligonucleotides (ON) that mimic the target DNA. We tested double stranded DNA dodecamers and hairpin-loop-forming oligonucleotides of different length. The integration biases with all target DNA structures tested were significantly influenced by changes in the resulting secondary ON structures.
    Trvalý link: http://hdl.handle.net/11104/0178483