Počet záznamů: 1

Comparison of HPLC/MS and MALDI-MS for characterizing triacylglycerols in insects: Specie-specific composition of lipids in fat bodies of bumblebee males

  1. 1.
    0332852 - UOCHB-X 2010 RIV NL eng J - Článek v odborném periodiku
    Kofroňová, Edita - Cvačka, Josef - Vrkoslav, Vladimír - Hanus, Robert - Jiroš, Pavel - Kindl, Jiří - Hovorka, Oldřich - Valterová, Irena
    Comparison of HPLC/MS and MALDI-MS for characterizing triacylglycerols in insects: Specie-specific composition of lipids in fat bodies of bumblebee males.
    Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. Roč. 877, č. 30 (2009), s. 3878-3884 ISSN 1570-0232
    Grant CEP: GA ČR GA203/09/0139; GA ČR GA203/09/1446; GA MŠk 2B06007
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: Bumblebee * fat body * triacylglycerols * HPLC/APCI-MS * MALDI-MS
    Kód oboru RIV: CC - Organická chemie
    Impakt faktor: 2.777, rok: 2009

    Two mass spectrometric methods for analysing triacylglycerols (HPLC/APCI-MS and MALDI-MS) were used and compared in terms of the relevance of the data for further biostatistical evaluation. While MALDI-MS is simpler and significantly faster, the time-consuming and labour-intensive HPLC/APCI-MS provides more complete information about the lipid components. The compositions of triacylglycerols in the fat bodies of males in 11 bumblebee species (Bombus terrestris, B. lucorum, B. lapidarius, B. pratorum, B. sylvarum, B. ruderatus, B. pomorum, B. subterraneus, B. campestris, B. bohemicus, and B. rupestris) were found to be species-specific.
    Trvalý link: http://hdl.handle.net/11104/0177980