Počet záznamů: 1

Association of ‘Candidatus Phytoplasma asteris’ with yellowing and phyllody of Plantago lanceolata

  1. 1.
    0331800 - BC-A 2010 RIV CZ eng J - Článek v odborném periodiku
    Fránová, Jana - Šimková, Marie
    Association of ‘Candidatus Phytoplasma asteris’ with yellowing and phyllody of Plantago lanceolata.
    Folia Microbiologica. Roč. 54, č. 5 (2009), s. 469-472 ISSN 0015-5632
    Grant CEP: GA AV ČR(CZ) 1QS500510558
    Výzkumný záměr: CEZ:AV0Z50510513
    Klíčová slova: symptoms * electron microscopy * molecular identification
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 0.978, rok: 2009

    Long plantains with symptoms resembling those associated with phytoplasma infection were observed in southern Bohemia. TEM showed phytoplasmas in the sieve cells of affected plants. Association of phytoplasmas with the disease was confirmed by PCR using phytoplasma-specific universal ribosomal primers R16F2n/R16R2. The restriction profiles obtained from digestion of the PCR products with endonucleases AluI, HhaI, and MseI showed that the phytoplasmas infecting long plantains in the Czech Republic were indistinguishable from those belonging to the aster yellows group (subgroup 16SrI-B). Sequence analysis of 1748 bp of the ribosomal operon indicated that the closest related phytoplasma was that associated with ‘Rehmannia glutinosa var. purpurea’, originating also in Bohemia. This is the first report of the natural occurrence of ‘Candidatus Phytoplasma asteris’ in plants of Plantago lanceolata.
    Trvalý link: http://hdl.handle.net/11104/0177222