Počet záznamů: 1
Complementation analysis of triple gene block of Potato virus S (PVS) revealed its capability to support systemic infection and aphid transmissibility of recombinant Potato virus X
0330919 - BC-A 2010 RIV NL eng J - Článek v odborném periodiku
Matoušek, Jaroslav - Schubert, J. - Dědič, P.
Complementation analysis of triple gene block of Potato virus S (PVS) revealed its capability to support systemic infection and aphid transmissibility of recombinant Potato virus X.
Virus Research. Roč. 146, č. 1 (2009), s. 81-88 ISSN 0168-1702
Grant CEP: GA MZe QF3109; GA AV ČR(CZ) 1QS500510558
Grant ostatní: EU Projekt FP7-REGPOT-2008-1 MOBITAG(XE) 229518
Výzkumný záměr: CEZ:AV0Z50510513
Klíčová slova: Recombinant virus * Virus host range * Potato virus X * Potato virus S
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 2.563, rok: 2009
Specific differences were found in triple gene block (TGB) sequences derived from isolates of ordinary Potato virus S (PVS-O) and Chenopodium-systemic (PVS-CS). In order to investigate functional divergence of PVS TGB variants, they were propagated in chimeric Potato virus X (PVX). Both PVS TGB variants partly complemented PVX TGB, but multiplied to lower titer than the wild-type PVX in N. benthamiana showing attenuated symptoms. From the propagation of recombinant PVX variants in different host and non-host plant species we assume there are additional determinants outside the TGB. Transmission by the aphid Myzus persicae in the recombinant virus was obtained by replacing PVX TGB by TGB from the PVS-CS isolate. These results show the potential function of Carlavirus TGB in aphid transmissibility and underlines the possible biological risks from certain recombinant virus variants.
Trvalý link: http://hdl.handle.net/11104/0176587