Počet záznamů: 1

The first occurrence of the cyanobacterial alkaloid toxin cylindrospermopsin in the Czech Republic as determined by immunochemical and LC/MS methods

  1. 1.
    0329609 - BU-J 2010 RIV GB eng J - Článek v odborném periodiku
    Bláhová, Lucie - Oravec, M. - Maršálek, Blahoslav - Šejnohová, Lenka - Šimek, Z. - Bláha, Luděk
    The first occurrence of the cyanobacterial alkaloid toxin cylindrospermopsin in the Czech Republic as determined by immunochemical and LC/MS methods.
    [První report o alkaloidním cylindrospermopsinu v ČR, analyza pomocí imunochemických metod a HPLC-MS.]
    Toxicon. Roč. 53, č. 5 (2009), s. 519-524 ISSN 0041-0101
    Grant CEP: GA MŠk 1M0571; GA ČR GA524/08/0496
    Výzkumný záměr: CEZ:AV0Z60050516
    Klíčová slova: cyanotoxin * cylindrospermopsin * methods of detection
    Kód oboru RIV: EF - Botanika
    Impakt faktor: 2.128, rok: 2009

    We report comparison of the immunochemical ELISA determination with the liquid chromatography/ mass spectrometry (LC/MS) method for CYN analyses in the samples from the Czech Republic, Central Europe. Results of the ELISA and LC/MS showed good qualitative agreement. However, concentrations determined by ELISA were systematically higher than concentrations determined by LC/MS. Differences between methods could be attributed to matrix effects (both in ELISA and LC/MS) or ELISA cross-reactivity with other unidentified derivatives of CYN. Our study demonstrates for the first time occurrence of CYN in the Central Europe.

    První celoplošná analýza obsahu cyanobakteriálního toxinu cylindrospermopsinu v povrchových vodách v České republice. Cylindrospermopsin analyzován ve vodné matrici imunochemickou (ELISA) a instrumentální metodou (LC-MS). Obě metody potvrdily jeho přítomnost ve třech lokalitách v ČR.
    Trvalý link: http://hdl.handle.net/11104/0175600