Počet záznamů: 1

Enrichment of rat oligodendrocyte progenitor cells by magnetic cell sorting

  1. 1.
    0328388 - UZFG-Y 2010 RIV NL eng J - Článek v odborném periodiku
    Čížková, D. - Čížek, M. - Nagyová, M. - Slovinská, L. - Novotná, I. - Jergová, S. - Radoňák, J. - Hlučilová, Jana - Vanický, I.
    Enrichment of rat oligodendrocyte progenitor cells by magnetic cell sorting.
    Journal of Neuroscience Methods. Roč. 184, č. 1 (2009), s. 88-94 ISSN 0165-0270
    Grant CEP: GA MŠk MEB0808108
    Grant ostatní:Agentúra na podporu výskumu a vývoja(SK) APVV-51002105; Agentúra na podporu výskumu a vývoja(SK) APVV SK-CZ-0045-07
    Výzkumný záměr: CEZ:AV0Z50450515
    Klíčová slova: Oligodendrocytes progenitors Lineage * Magnetic separation
    Kód oboru RIV: FH - Neurologie, neurochirurgie, neurovědy
    Impakt faktor: 2.295, rok: 2009

    In the present study we have used magnetic cell sorting (MACs) technology to generate enriched oligodendroglial cell populations from the embryonic (E16) rat spinal cord. Target cells were separated by positive selection, using specific A2B5 antibody-labeled MicroBeads achieving optimal recovery and high purity of pro-oligodendroglial cells. Based on immunocytochemical analyses for oligodendroglial developmental markers (A2B5, NG2, RIP and MBP) we were able to characterize and quantify oligodendroglial progenitors (OPCs) and mature oligodendroglial cells in: (i) unseparated heterogeneous population of NSCs, or in (ii) antigen–antibody separated NSCs. Our results showed that MACs technology enable us to gain enriched OPCs from heterogeneous population of spinal NSCs, resulting in a 58–61% of mature oligodendrocytes content (MBP+, RIP+) in comparison to 6–12% of oligodendroglial cells acquired from unseparated population. In addition, the enriched OPCs could be cultured in vitro for several >8 passages, giving rise to a high number of newly formed spheres, as well as high expansion potential. These experiments indicate that MACs technology provide a feasible approach for experimental cell enrichment of desired oligodendroglial progeny, which may be used in future trials for cell-based therapies to treat spinal cord injury.
    Trvalý link: http://hdl.handle.net/11104/0174715