Počet záznamů: 1

The strand transfer oligonucleotide inhibitors of HIV-integrase

  1. 1.
    0327524 - UOCHB-X 2010 RIV GB eng J - Článek v odborném periodiku
    Snášel, Jan - Rosenberg, Ivan - Pačes, Ondřej - Pichová, Iva
    The strand transfer oligonucleotide inhibitors of HIV-integrase.
    Journal of Enzyme Inhibition and Medicinal Chemistry. Roč. 24, č. 1 (2009), s. 241-246 ISSN 1475-6366
    Grant CEP: GA MŠk 1M0508; GA ČR GA203/05/0827; GA ČR GP203/05/P557; GA AV ČR IAA4055304
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: HIV-1 integrase * inhibition * phosphonate oligonucleotides
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 1.496, rok: 2009

    We have designed and synthesized oligonucleotides (ONs) of various lengths that mimic the processed HIV-1 U5 terminus of the proviral long terminal repeat (LTR) and are ended by 2‘-deoxyadenosine containing a 3‘-O-phosphonomethyl group for inhibition of HIV-1 integrase. The duplex stability of phosphonomethyl ONs was increased by covalent linkage of the modified strand with its complementary strand by a triethylene glycol loop (TEG). Modified ONs containing up to 10 bases inhibited in vitro the strand transfer reaction catalyzed by HIV-1 integrase at nanomolar concentrations.
    Trvalý link: http://hdl.handle.net/11104/0174316