Počet záznamů: 1

Glycosidases: a key to tailored carbohydrates

  1. 1.
    0327136 - MBU-M 2010 RIV GB eng J - Článek v odborném periodiku
    Bojarová, Pavla - Křen, Vladimír
    Glycosidases: a key to tailored carbohydrates.
    Trends in Biotechnology. Roč. 27, č. 4 (2009), s. 199-209 ISSN 0167-7799
    Grant CEP: GA AV ČR IAA400200503; GA MŠk(CZ) LC06010; GA ČR GP203/09/P024
    Výzkumný záměr: CEZ:AV0Z50200510
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 6.909, rok: 2009

    In recent years, carbohydrate-processing enzymes have become the enzymes of choice in many applications thanks to their stereoselectivity and efficiency. This review presents recent developments in glycosidasecatalyzed synthesis via two complementary approaches: the use of wild-type enzymes with engineered substrates, and mutant glycosidases. Genetic engineering has recently produced glucuronyl synthases, an inverting xylosynthase and the first mutant endo-b-N-acetylglucosaminidase. A thorough selection of enzyme strains and aptly modified substrates have resulted in rare glycostructures, such as N-acetyl-b-galactosaminuronates, b1,4-linked mannosides and a1,4-linked galactosides. The efficient selection of mutant enzymes is facilitated by high-throughput screening assays involving the co-expression of coupled enzymes or chemical complementation
    Trvalý link: http://hdl.handle.net/11104/0174034