Počet záznamů: 1

Analysis of insect triacylglycerols using liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry

  1. 1.
    0326454 - UOCHB-X 2010 RIV DE eng J - Článek v odborném periodiku
    Kofroňová, Edita - Cvačka, Josef - Jiroš, Pavel - Sýkora, D. - Valterová, Irena
    Analysis of insect triacylglycerols using liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry.
    European Journal of Lipid Science and Technology. Roč. 111, č. 5 (2009), s. 519-525 ISSN 1438-7697
    Grant CEP: GA AV ČR IAA4055403; GA MŠk 2B06007
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: atmospheric pressure chemical ionization * bumblebees * fat body * NARP-HPLC
    Kód oboru RIV: CC - Organická chemie
    Impakt faktor: 1.831, rok: 2009

    An HPLC non-aqueous reversed-phase separation system was adapted for analyzing insect triacylglycerols (TAG). The method uses two conventional Nova-Pak C18 columns connected in series, for a total length of 45 cm. The mobile phase gradient is mixed from acetonitrile and 2-propanol, and the flow rate is programmed from 1.0 to 0.7 mL/min. TAG are detected by atmospheric pressure chemical ionization mass spectrometry. The method ensures efficient separation of isomers and analysis of high-molecular weight TAG with equivalent chain lengths up to 72. The method performance is demonstrated on analysis of TAG isolated from the fat body of the bumblebee Bombus lucorum.
    Trvalý link: http://hdl.handle.net/11104/0173556