Search results
- 1.0523128 - BTÚ 2020 RIV NL eng J - Journal Article
Švec, David - Tichopád, A. - Novosadová, Vendula - Pfaffl, M. W. - Kubista, Mikael
How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments.
Biomolecular Detection and Quantification. Roč. 3, March 01 (2015), s. 9-16. ISSN 2214-7535
R&D Projects: GA ČR GA13-02154S; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:86652036
Keywords : Amplification efficiency * Dilution series * qpcr * QPCR assay validation * Real-time quantitative PCR * Standard curve
OECD category: Biochemistry and molecular biology
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S2214753515000169?via%3Dihub
Permanent Link: http://hdl.handle.net/11104/0307524 - 2.0473393 - BTÚ 2017 RIV NL eng J - Journal Article
Bjorkman, J. - Švec, David - Kubista, Mikael - Sjöback, R.
Differential amplicons (ΔAmp)—a new molecular method to assess RNA integrity.
Biomolecular Detection and Quantification. Roč. 6, Jan 2016 (2016), s. 4-12. ISSN 2214-7535
R&D Projects: GA ČR GA13-02154S; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:86652036
Keywords : RNA integrity * RNA quality * Endogenous RNase resistant marker
Subject RIV: EB - Genetics ; Molecular Biology
Permanent Link: http://hdl.handle.net/11104/0270532 - 3.0457855 - BTÚ 2016 RIV GB eng J - Journal Article
Korenková, Vlasta - Scott, J. - Novosadová, Vendula - Jindřichová, Marie - Langerová, Lucie - Švec, David - Šídová, Monika - Sjoback, R.
Pre-amplification in the context of high-throughput qPCR gene expression experiment.
B M C Molecular Biology. Roč. 16, č. 5 (2015). ISSN 1471-2199. E-ISSN 1471-2199
R&D Projects: GA ČR(CZ) GAP304/12/1585; GA ČR(CZ) GA15-08239S; GA ČR GA13-02154S; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:86652036
Keywords : High-throughput qPCR * Gene expression * Exponential pre-amplification
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.500, year: 2015
Permanent Link: http://hdl.handle.net/11104/0258216 - 4.0453048 - ÚEM 2016 RIV US eng J - Journal Article
Džamba, Dávid - Honsa, Pavel - Valný, Martin - Kriška, Ján - Valihrach, Lukáš - Novosadová, Vendula - Kubista, Mikael - Anděrová, Miroslava
Quantitative Analysis of Glutamate Receptors in Glial Cells from the Cortex of GFAP/EGFP Mice Following Ischemic Injury: Focus on NMDA Receptors.
Cellular and Molecular Neurobiology. Roč. 35, č. 8 (2015), s. 1187-1202. ISSN 0272-4340. E-ISSN 1573-6830
R&D Projects: GA ČR(CZ) GBP304/12/G069; GA ČR GA13-02154S; GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT(CZ) EE2.3.30.0045
Institutional support: RVO:68378041 ; RVO:86652036
Keywords : astrocytes * NG2 glia * MCAo * Single-cell RT-qPCR * Calcium imaging
Subject RIV: ED - Physiology; EI - Biotechnology ; Bionics (BTO-N)
Impact factor: 2.328, year: 2015
Permanent Link: http://hdl.handle.net/11104/0253948 - 5.0436669 - ÚEM 2015 RIV US eng J - Journal Article
Anděrová, Miroslava - Benešová, Jana - Mikešová, Michaela - Džamba, Dávid - Honsa, Pavel - Kriška, Ján - Butenko, Olena - Novosadová, Vendula - Valihrach, Lukáš - Kubista, Mikael - Dmytrenko, L. - Cicanič, M. - Vargová, L.
Altered Astrocytic Swelling in the Cortex of α-Syntrophin-Negative GFAP/EGFP Mice.
PLoS ONE. Roč. 9, č. 11 (2014), e113444. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA ČR GA13-02154S; GA ČR(CZ) GBP304/12/G069; GA MŠMT(CZ) ED1.1.00/02.0109
Grant - others:GA MŠk(CZ) CZ.1.07/2.3.00/30.0045
Institutional support: RVO:68378041 ; RVO:86652036
Keywords : astrocyte volume regulation * aquaporines * extracellular space
Subject RIV: FH - Neurology
Impact factor: 3.234, year: 2014
Permanent Link: http://hdl.handle.net/11104/0240347 - 6.0435512 - ÚEM 2016 RIV US eng J - Journal Article
Minieri, L. - Pivoňková, Helena - Harantová, Lenka - Anděrová, Miroslava - Ferroni, S.
Intracellular Na+ inhibits volume regulated anion channel in rat cortical astrocytes.
Journal of Neurochemistry. Roč. 132, č. 3 (2015), s. 286-300. ISSN 0022-3042. E-ISSN 1471-4159
R&D Projects: GA ČR(CZ) GBP304/12/G069; GA ČR GA13-02154S
Institutional support: RVO:68378041
Keywords : volume-regulated anion channels * intracellular sodium * cell volume
Subject RIV: FH - Neurology
Impact factor: 3.842, year: 2015
Permanent Link: http://hdl.handle.net/11104/0243597 - 7.0432501 - BTÚ 2015 RIV GB eng J - Journal Article
Stahlberg, A. - Kubista, Mikael
The workflow of single-cell expression profiling using quantitative real-time PCR.
Expert Review of Molecular Diagnostics. Roč. 14, č. 3 (2014), s. 323-331. ISSN 1473-7159. E-ISSN 1744-8352
R&D Projects: GA ČR GA13-02154S; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:86652036
Keywords : single-cell workflow * gene expression profiling * RT-qPCR
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.516, year: 2014
Permanent Link: http://hdl.handle.net/11104/0236884 - 8.0431253 - ÚEM 2015 RIV US eng J - Journal Article
Honsa, Pavel - Pivoňková, Helena - Harantová, Lenka - Butenko, Olena - Kriška, Ján - Džamba, Dávid - Rusňáková, Vendula - Valihrach, Lukáš - Kubista, Mikael - Anděrová, Miroslava
Increased expression of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels in reactive astrocytes following ischemia.
Glia. Roč. 62, č. 12 (2014), s. 2004-2021. ISSN 0894-1491. E-ISSN 1098-1136
R&D Projects: GA ČR GA13-02154S; GA ČR(CZ) GBP304/12/G069; GA MŠMT(CZ) ED1.1.00/02.0109
Grant - others:GA MŠk(CZ) CZ.1.07/2.3.00/30.0045
Institutional support: RVO:68378041 ; RVO:86652036
Keywords : Astrocytes * focal and global cerebral ischemia * HCN channels
Subject RIV: FH - Neurology
Impact factor: 6.031, year: 2014
Permanent Link: http://hdl.handle.net/11104/0239346 - 9.0398761 - BTÚ 2014 RIV US eng J - Journal Article
Rusňáková, Vendula - Honsa, Pavel - Džamba, Dávid - Stahlberg, A. - Kubista, Mikael - Anděrová, Miroslava
Heterogeneity of Astrocytes: From Development to Injury - Single Cell Gene Expression.
PLoS ONE. Roč. 8, č. 8 (2013), e69734. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA ČR GA13-02154S
Institutional research plan: CEZ:AV0Z50520701; CEZ:AV0Z50390703
Keywords : Single cell expression profiling * astrocytes * GenEx
Subject RIV: EB - Genetics ; Molecular Biology; FH - Neurology (UEM-P)
Impact factor: 3.534, year: 2013
Permanent Link: http://hdl.handle.net/11104/0226963