Search results
- 1.0507540 - FGÚ 2020 RIV NL eng J - Journal Article
Janáček, Jiří - Brejchová, Jana - Svoboda, Petr
Determination of delta-opioid receptor molecules mobility in living cells plasma membrane by novel method of FRAP analysis.
Biochimica Et Biophysica Acta-Biomembranes. Roč. 1861, č. 7 (2019), s. 1364-1354. ISSN 0005-2736. E-ISSN 1879-2642
R&D Projects: GA ČR(CZ) GA17-05903S; GA MŠMT(CZ) LM2015062; GA MŠMT(CZ) EF16_013/0001775
Research Infrastructure: Czech-BioImaging - 90062
Institutional support: RVO:67985823
Keywords : FRAP * adaptive ROI approach * delta-Opioid receptor-eYFP mobility * plasma membrane * cholesterol depletion * cholesterol replenishment
OECD category: Biochemical research methods
Impact factor: 3.411, year: 2019
Method of publishing: Open access with time embargo
https://doi.org/10.1016/j.bbamem.2019.04.012
Permanent Link: http://hdl.handle.net/11104/0298518 - 2.0492456 - FGÚ 2019 RIV US eng J - Journal Article
Vošahlíková, Miroslava - Ujčíková, Hana - Hloušková, Martina - Musil, Stanislav - Roubalová, Lenka - Alda, M. - Svoboda, Petr
Induction of oxidative stress by long-term treatment of live HEK293 cells with therapeutic concentration of lithium is associated with down-regulation of delta-opioid receptor amount and function.
Biochemical Pharmacology. Roč. 154, Aug (2018), s. 452-463. ISSN 0006-2952. E-ISSN 1873-2968
R&D Projects: GA ČR(CZ) GA17-07070S; GA ČR(CZ) GA17-05903S
Institutional support: RVO:67985823 ; RVO:68081715
Keywords : lithium * HEK293 cells * delta-opioid receptor * G protein * oxidative stress
OECD category: Physiology (including cytology); Analytical chemistry (UIACH-O)
Impact factor: 4.825, year: 2018
Permanent Link: http://hdl.handle.net/11104/0285989 - 3.0463372 - FGÚ 2017 RIV US eng J - Journal Article
Brejchová, Jana - Vošahlíková, Miroslava - Roubalová, Lenka - Parenti, M. - Mauri, M. - Chernyavskiy, Oleksandr - Svoboda, Petr
Plasma membrane cholesterol level and agonist-induced internalization of delta-opioid receptors; colocalization study with intracellular membrane markers of Rab family
.
Journal of Bioenergetics and Biomembranes. Roč. 48, č. 4 (2016), s. 375-396. ISSN 0145-479X. E-ISSN 1573-6881
R&D Projects: GA ČR(CZ) GAP207/12/0919
Institutional support: RVO:67985823
Keywords : cholesterol * plasma membrane * delta-opioid receptor * internalization * Rab proteins
Subject RIV: CE - Biochemistry
Impact factor: 2.576, year: 2016
Permanent Link: http://hdl.handle.net/11104/0262569 - 4.0447525 - FGÚ 2016 RIV US eng J - Journal Article
Roubalová, Lenka - Vošahlíková, Miroslava - Brejchová, Jana - Sýkora, Jan - Rudajev, Vladimír - Svoboda, Petr
High Efficacy but Low Potency of delta-Opioid Receptor-G Protein Coupling in Brij-58-Treated, Low-Density Plasma Membrane Fragments.
PLoS ONE. Roč. 10, č. 8 (2015), e0135664. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA ČR(CZ) GAP207/12/0919
Institutional support: RVO:67985823 ; RVO:61388955
Keywords : delta - opioid receptor * G protein coupling * detergent * efficacy * potency
Subject RIV: CE - Biochemistry; CF - Physical ; Theoretical Chemistry (UFCH-W)
Impact factor: 3.057, year: 2015
Permanent Link: http://hdl.handle.net/11104/0249343 - 5.0428435 - FGÚ 2015 RIV DE eng J - Journal Article
Vošahlíková, Miroslava - Jurkiewicz, Piotr - Roubalová, Lenka - Hof, Martin - Svoboda, Petr
High- and low-affinity sites for sodium in delta-OR-G(i)1 alpha (Cys(351)-Ile(351)) fusion protein stably expressed in HEK293 cells; functional significance and correlation with biophysical state of plasma membrane.
Naunyn-Schmiedeberg's Archives of Pharmacology. Roč. 387, č. 5 (2014), s. 487-502. ISSN 0028-1298. E-ISSN 1432-1912
R&D Projects: GA ČR(CZ) GAP207/12/0919; GA ČR(CZ) GBP304/12/G069
Institutional support: RVO:67985823 ; RVO:61388955
Keywords : delta - opioid receptor * monovalent ions * agonist and antagonist binding * [35S]GTPγS binding * membrane biophysics * Laurdan fluorescence
Subject RIV: CE - Biochemistry
Impact factor: 2.471, year: 2014
Permanent Link: http://hdl.handle.net/11104/0233793 - 6.0370263 - FGÚ 2012 RIV CZ eng J - Journal Article
Vošahlíková, Miroslava - Svoboda, Petr
The influence of monovalent cations on trimeric G protein Gi1alfa activity in HEK293 cells stably expressing DOR-Gi1alfa (Cys351-Ile351) fusion protein.
Physiological Research. Roč. 60, č. 3 (2011), s. 541-547. ISSN 0862-8408. E-ISSN 1802-9973
R&D Projects: GA AV ČR(CZ) IAA500110606; GA MŠMT(CZ) LC554; GA ČR(CZ) GD305/08/H037
Institutional research plan: CEZ:AV0Z50110509
Keywords : delta-opioid receptor (DOR) * monovalent ions * G(i)1alfa protein
Subject RIV: CE - Biochemistry
Impact factor: 1.555, year: 2011
Permanent Link: http://hdl.handle.net/11104/0204113 - 7.0365285 - FGÚ 2012 RIV NL eng J - Journal Article
Brejchová, Jana - Sýkora, Jan - Dlouhá, Kateřina - Roubalová, Lenka - Ostašov, Pavel - Vošahlíková, Miroslava - Hof, Martin - Svoboda, Petr
Fluorescence spectroscopy studies of HEK293 cells expressing DOR-Gi1alfa fusion protein; the effect of cholesterol depletion.
Biochimica Et Biophysica Acta-Biomembranes. Roč. 1808, č. 12 (2011), s. 2819-2829. ISSN 0005-2736. E-ISSN 1879-2642
R&D Projects: GA MŠMT(CZ) LC06063; GA MŠMT(CZ) LC554; GA ČR(CZ) GD305/08/H037
Grant - others:GA ČR(CZ) GAP208/10/1090
Institutional research plan: CEZ:AV0Z50110509; CEZ:AV0Z40400503
Keywords : plasma membrane * cholesterol depletion * fluorescence spectroscopy * hydrophobic membrane interior * delta-opioid receptor (DOR), * G protein
Subject RIV: BO - Biophysics
Impact factor: 3.990, year: 2011
Permanent Link: http://hdl.handle.net/11104/0200565 - 8.0054839 - FGÚ 2008 ES eng A - Abstract
Novotný st., Jiří - Bouřová, Lenka - Svoboda, Petr
The intact structure of membrane domains is important for functional interaction of delta-opioid receptors with Gi proteins.
[Intaktní struktura membránových domén je důležitá pro funkční interakci delta-opioidních receptorů s Gi proteiny.]
European opioid conference. Salamanca, 2006. P21-P21.
[European opioid conference /6./. 19.04.2006-21.04.2006, Salamanca]
Keywords : membrane domains * delta-opioid receptor * G protein * GPCR
Subject RIV: CE - Biochemistry
Permanent Link: http://hdl.handle.net/11104/0142766 - 9.0054829 - FGÚ 2008 US eng A - Abstract
Rudajev, Vladimír - Stöhr, Jiří - Bouřová, Lenka - Novotný st., Jiří - Svoboda, Petr
Different behaviour of GPCRs when exposed to non-ionic detergents. GPCR/G protein coupling in detergent-resistant membrane domains.
[Rozdílné chování GPCR vystavených neiontovým detergentům. Spřažení GPCR a G proteinů v detergent-rezistentních doménách.]
Keystone symposia on lipids rafts and cell function - 2006 abstracts book. 2006. s. 70-70.
[Keystone symposia on lipid rafts and cell function. 23.03.2006-28.03.2006, Steamboat Springs]
R&D Projects: GA MŠMT(CZ) LC554
Institutional research plan: CEZ:AV0Z50110509
Keywords : GPCR * G protein * detergent-resistent membrane domains * GABAB * delta-opioid receptor
Subject RIV: CE - Biochemistry
Permanent Link: http://hdl.handle.net/11104/0142760