Testing of Fibrous Membranes with Different Scaffold Morphology for Development of 3D Skin Model

Vera  Sovková; Karolína Vocetková; Michala  Rampichová; Eva Filová

doi:10.4028/www.scientific.net/KEM.834.148

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HomeKey Engineering MaterialsKey Engineering Materials Vol. 834Testing of Fibrous Membranes with Different...

Testing of Fibrous Membranes with Different Scaffold Morphology for Development of 3D Skin Model

Abstract:

Replacement of in vivo testing using advanced 3D constructs is an important challenge in tissue engineering applications. The cell culture material should be biocompatible and should mimic the natural microenvironment of the existing tissue. Nanofibrous scaffolds prepared by electrospinning from biocompatible polymers have suitable properties for cell culture in a 3D environment. Thanks to the high volume-to-surface ratio, controlled porous structure with high pore interconnection and microarchitecture in the nanoscale range, nanofibers are in the foreground of interest. We tested membranes with different topography with keratinocyte and fibroblast cell lines. Fibroblast showed stable growth with no difference among the scaffolds. On the other hand, keratinocytes preferred scaffolds with nanofiber morphology.

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Periodical:

Key Engineering Materials (Volume 834)

Pages:

148-154

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.834.148

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Online since:

March 2020

Authors:

Vera Sovková*, Karolína Vocetková, Michala Rampichová, Eva Filová

Keywords:

Fibroblasts, Keratinocytes, Scaffold, Skin

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* - Corresponding Author

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