Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties
Abstract
:1. Introduction
2. Results
2.1. Effects of Light Regimes on Growth
2.2. Light-Induced Accumulation of UV-Absorbing Substances
2.3. Phenolic Profile of Basil Leaves under Different Light Regimes
2.4. Antioxidant Response
2.5. H2O2 Accumulation in Leaves
3. Discussion
3.1. Contrasting Effects of Sunlight and UV-B/Moderate Light
3.2. The Epidermal UV-Attenuation Capacity in Green vs. Purple Leaves
3.3. Accumulation of Asc, HCAs, and Flavonoids Is Affected by the Different UV-B/PAR Ratio
3.4. Differential Photomorphogenic and Antioxidative Response of Three Basil Varieties
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. Phenolics Determination with HPLC-DAD
4.3. Measurement of Reduced Ascorbate
4.4. Determination of POD Activity
4.5. Antioxidant Capacity
4.6. Determination (Visualization) of H2O2 in Basil Leaves
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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GB | CB | PB | ||||
---|---|---|---|---|---|---|
GH | OP | GH | OP | GH | OP | |
FW (g) | 19.00 ± 1.78 | 12.33 ± 0.62 * | 8.67 ± 0.94 | 12.00 ± 1.47 | 9.00 ± 0.82 | 8.67 ± 0.94 |
DW (g) | 1.56 ± 0.13 | 1.80 ± 0.08 | 0.86 ± 0.05 | 1.65 ± 0.21 * | 0.53 ± 0.02 | 0.91 ± 0.06 |
FW/DW | 12.18 ± 0.17 | 6.88 ± 0.36 *** | 10.16 ± 0.39 | 7.27 ± 0.06 ** | 16.88 ± 0.97 | 9.47 ± 0.47 ** |
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Milić Komić, S.; Živanović, B.; Dumanović, J.; Kolarž, P.; Sedlarević Zorić, A.; Morina, F.; Vidović, M.; Veljović Jovanović, S. Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties. Int. J. Mol. Sci. 2023, 24, 15350. https://doi.org/10.3390/ijms242015350
Milić Komić S, Živanović B, Dumanović J, Kolarž P, Sedlarević Zorić A, Morina F, Vidović M, Veljović Jovanović S. Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties. International Journal of Molecular Sciences. 2023; 24(20):15350. https://doi.org/10.3390/ijms242015350
Chicago/Turabian StyleMilić Komić, Sonja, Bojana Živanović, Jelena Dumanović, Predrag Kolarž, Ana Sedlarević Zorić, Filis Morina, Marija Vidović, and Sonja Veljović Jovanović. 2023. "Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties" International Journal of Molecular Sciences 24, no. 20: 15350. https://doi.org/10.3390/ijms242015350