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In Vitro Reconstitution of Molecular Motor-Driven Mitochondrial Transport

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Axonal Transport

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2431))

Abstract

Intracellular trafficking of organelles driven by molecular motors underlies essential cellular processes. Mitochondria, the powerhouses of the cell, are one of the major cargoes of molecular motors. Efficient distribution of mitochondria ensures cellular fitness while defects in this process contribute to severe pathologies, such as neurodegenerative diseases. Reconstitution of the mitochondrial microtubule-based transport in vitro in a bottom-up approach provides a powerful tool to investigate the mitochondrial trafficking machinery in a controlled environment in the absence of complex intracellular interactions. In this chapter, we describe the procedures for achieving such reconstitution of mitochondrial transport.

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Authors and Affiliations

  1. Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, Prague West, Czech Republic

    Verena Puttrich, Jakub Rohlena, Marcus Braun & Zdenek Lansky

Authors
  1. Verena Puttrich
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  2. Jakub Rohlena
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  3. Marcus Braun
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  4. Zdenek Lansky
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Corresponding author

Correspondence to Zdenek Lansky .

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Editors and Affiliations

  1. Department of Basic and Clinical Neurosciences, Maurice Wohl Clinical Neuroscience Institute, Institute of Psychiatry, Psychology and Neuroscience, King’s College London, London, UK

    Alessio Vagnoni

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Puttrich, V., Rohlena, J., Braun, M., Lansky, Z. (2022). In Vitro Reconstitution of Molecular Motor-Driven Mitochondrial Transport. In: Vagnoni, A. (eds) Axonal Transport. Methods in Molecular Biology, vol 2431. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1990-2_28

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  • DOI: https://doi.org/10.1007/978-1-0716-1990-2_28

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1989-6

  • Online ISBN: 978-1-0716-1990-2

  • eBook Packages: Springer Protocols

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