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Biosynthetic Gene Cluster Analysis in Actinobacterial Genus Streptomyces

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Methods in Actinobacteriology

Part of the book series: Springer Protocols Handbooks ((SPH))

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Abstract

Secondary metabolites offer a wide variety of biologically active compounds with great potential in medicine, agriculture, or livestock. With the advance of computational techniques and genome sequencing availability, laborious screening of microbial products can be replaced by more efficient genome mining using extensive genome databases available online. Rapidly developing bioinformatic tools, such as antiSMASH, enable researches to effectively explore the genome of many organisms including plants, fungi, and bacteria and identify biosynthetic gene clusters potentially encoding novel bioactive compounds. In this chapter, we provide a step-by-step guide of using the antiSMASH online tool to identify gene clusters potentially encoding for secondary metabolites in the genome of the model strain Streptomyces griseus NBRC 13350.

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Author information

Authors and Affiliations

  1. Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic

    Markéta Macho & Jihen Thabet

  2. Laboratory of Algal Biotechnology, Centre Algatech, Institute of Microbiology of the Czech Academy of Sciences, Třeboň, Czech Republic

    Markéta Macho, Daniela Ewe, Jihen Thabet, Avik Banerjee, Kumar Saurav & Subhasish Saha

  3. Department of Biotechnology, Himachal Pradesh University, Shimla, India

    Vishal Ahuja

Authors
  1. Markéta Macho
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  2. Daniela Ewe
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  3. Vishal Ahuja
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  4. Jihen Thabet
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  5. Avik Banerjee
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  6. Kumar Saurav
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  7. Subhasish Saha
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Corresponding author

Correspondence to Subhasish Saha .

Editor information

Editors and Affiliations

  1. Department of Microbiology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India

    Dhanasekaran Dharumadurai

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Macho, M. et al. (2022). Biosynthetic Gene Cluster Analysis in Actinobacterial Genus Streptomyces. In: Dharumadurai, D. (eds) Methods in Actinobacteriology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1728-1_33

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  • DOI: https://doi.org/10.1007/978-1-0716-1728-1_33

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1727-4

  • Online ISBN: 978-1-0716-1728-1

  • eBook Packages: Springer Protocols

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