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Kupffer Cells pp 65–73Cite as

Glucan-Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Kupffer Cells in Mouse Liver

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2164))

Abstract

Here, we describe a protocol to prepare and administer glucan-encapsulated RNAi particles (GeRPs), for specific delivery of siRNA and subsequent gene silencing in Kupffer cells (KCs) in mice. This technology is based on baker’s yeast and allows gene manipulation in macrophages in a tissue-specific manner depending on the route of administration and the model that is used. GeRP administered by intravenous injection in mice are delivered to KCs. Therefore, using the GeRP technology to silence genes provides a unique method to study the function of factors expressed by KCs in the regulation of liver function.

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Acknowledgments

This work was supported by research grants from the Czech Science Foundation GACR 20-03586S and the START UP program from Institute of Physiology, the Czech Academy of Sciences.

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Authors and Affiliations

  1. Department of Molecular Physiology of Bone, Institute of Physiology Czech Academy of Sciences, Prague, Czech Republic

    Michaela Tencerova

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  1. Michaela Tencerova
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Correspondence to Michaela Tencerova .

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Editors and Affiliations

  1. Department of Medicine, Integrated Cardio Metabolic Center, Karolinska Institutet, Huddinge, Sweden

    Myriam Aouadi

  2. Department of Medicine, Integrated Cardio Metabolic Center, Karolinska Institutet, Huddinge, Sweden

    Valerio Azzimato

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Tencerova, M. (2020). Glucan-Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Kupffer Cells in Mouse Liver. In: Aouadi, M., Azzimato, V. (eds) Kupffer Cells. Methods in Molecular Biology, vol 2164. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0704-6_8

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  • DOI: https://doi.org/10.1007/978-1-0716-0704-6_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0703-9

  • Online ISBN: 978-1-0716-0704-6

  • eBook Packages: Springer Protocols

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