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Capillary Electrophoresis-Mass Spectrometry

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2531)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
  2. Evaluation of Prototype CE-MS Interfaces

    • Sabrina Ferré, Julien Boccard, Serge Rudaz, Víctor González-Ruiz
    Pages 1-13
  3. Protocol for Etching Bare-Fused Silica Capillaries for Sheathless Capillary Electrophoresis–Mass Spectrometry Coupling

    • Clarisse Gosset-Erard, Jérémie Giorgetti, Michael Biacchi, Frédéric Aubriet, Emmanuelle Leize-Wagner, Patrick Chaimbault et al.
    Pages 49-59
  4. Successive Multiple Ionic-Polymer Layer Coatings for Intact Protein Analysis by Capillary Zone Electrophoresis–Mass Spectrometry: Application to Hemoglobin Analysis

    • Liesa Salzer, Alexander Stolz, Laura Dhellemmes, Alisa Höchsmann, Laurent Leclercq, Hervé Cottet et al.
    Pages 69-76
  5. On-line Immobilized Enzyme Microreactor Capillary Zone Electrophoresis–Mass Spectrometry for Peptide Mapping

    • Roger Pero-Gascon, Laura Pont, Estela Giménez, Victoria Sanz-Nebot, Fernando Benavente
    Pages 77-91
  6. Assessment of Macro- and Microheterogeneity of Monoclonal Antibodies Using Capillary Zone Electrophoresis Hyphenated with Mass Spectrometry

    • Christoph Gstöttner, Rob Haselberg, Manfred Wuhrer, Govert W. Somsen, Elena Domínguez-Vega
    Pages 125-142
  7. High-Sensitivity Glycoproteomic Analysis of Biological Samples by CZE-ESI-MS

    • W. Wang, G. S. M. Lageveen-Kammeijer
    Pages 143-162
  8. In-Source Fragmentation for the Identification of Compounds by CE-ESI-TOF in Human Plasma. l-Proline as Case Study

    • Maricruz Mamani-Huanca, Ana Gradillas, Ángeles López-Gonzálvez, Coral Barbas
    Pages 185-202
  9. Applications of Capillary Electrophoresis-Mass Spectrometry to Chiral Analysis

    • Elena Sánchez-López, María Luisa Marina
    Pages 211-225
  10. CE Coupled to ICP-MS and Single Particle ICP-MS for Nanoparticle Analysis

    • Darya Mozhayeva, Carsten Engelhard
    Pages 243-257
  11. Back Matter

    Pages 259-260

About this book

This volume details aspects and applications of interfacing capillary electrophoresis (CE) with mass spectrometry (MS). Chapters guide readers through approaches based on different types of CE-MS interfaces such as (nano)sheath liquid, porous tip, and liquid junction, as well as various capillary coatings, and a broad range of applications including several top-down and bottom-up proteomic approaches. Additionally, a list of analyte targets was provided consisting of amphetamines, antibiotics, carbohydrates (including glycosaminoglycans and glycopeptides), enantiomers, extracellular matrix metabolites, monoclonal antibodies, and nanoparticles, and therefore covers numerous fields of applications such as pharmaceutical, biomedical, food, agrochemical, and environmental analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Capillary Electrophoresis-Mass Spectrometry: Methods and Protocols aims to provide highly valuable information for both beginners and experts in the field be it students, technical staff, and scientists.


Editors and Affiliations

  • Aalen University, Aalen, Germany

    Christian Neusüß, Kevin Jooß

Bibliographic Information

  • Book Title: Capillary Electrophoresis-Mass Spectrometry

  • Book Subtitle: Methods and Protocols

  • Editors: Christian Neusüß, Kevin Jooß

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2493-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2492-0Published: 09 August 2022

  • Softcover ISBN: 978-1-0716-2495-1Published: 10 August 2023

  • eBook ISBN: 978-1-0716-2493-7Published: 08 August 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 260

  • Number of Illustrations: 1 b/w illustrations, 4 illustrations in colour

  • Topics: Mass Spectrometry, Biochemistry, general

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access