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Biphasic optimization approach for maximization of lipid production by the microalga Chlorella pyrenoidosa

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Abstract

The aim of the study was to identify the optimum cultivation conditions for the microalgal growth and lipid production of the oleaginous microalga Chlorella pyrenoidosa Chick (IPPAS C2). Moreover, an appropriate NO3− concentration in the cultivation medium for maximized lipid accumulation was determined. The experimental design involved a biphasic cultivation strategy with an initial biomass accumulating phase under optimized light (400 μmol/m2 per s), temperature (25 °C), and elevated CO2 concentration in the air mixture (3%), followed by a mid-elevated CO2 concentration (0.5%) for lipid induction. The highest lipid yields of 172.47 ± 18.1 and 179.65 ± 25.4 mg/L per day were detected for NO3− concentrations of 100 and 150 mg/L. The optimization approach presented here led not only to the maximization of lipid yield but also to the development of a biphasic cultivation strategy easily applicable to the cultivation process without the necessity for algal cell harvesting between the first and second cultivation phases.

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Funding

This study was funded by the Ministry of Education, Youth and Sports of the Czech Republic (OP RDE grant number CZ.02.1.01/0.0/0.0/16_026/0008413 “Strategic Partnership for Environmental Technologies and Energy Production” and the National Sustainability Program I (NPU I) grant number LO1415).

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Authors and Affiliations

  1. Global Change Research Institute, Academy of Sciences of the Czech Republic, Bělidla 986/4a, 603 00, Brno, Czech Republic

    Kateřina Sukačová, Diana Búzová & Jan Červený

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  1. Kateřina Sukačová
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  2. Diana Búzová
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  3. Jan Červený
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All authors contributed to the study conception and design. All authors read and approved the final manuscript.

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Correspondence to Kateřina Sukačová.

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Sukačová, K., Búzová, D. & Červený, J. Biphasic optimization approach for maximization of lipid production by the microalga Chlorella pyrenoidosa. Folia Microbiol 65, 901–908 (2020). https://doi.org/10.1007/s12223-020-00800-w

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  • DOI: https://doi.org/10.1007/s12223-020-00800-w

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